Molecular biology and tissue culture programs are operated in DSIR recognized 8,000 square feet lab facility of global standards. Our areas of focus include plant molecular biology, plant tissue culture and plant diagnostics.

Our Services:

• Nucleic acid isolation-Plant genomic DNA/total RNA/mRNA
• Target specific protein quantification by ELISA

• Marker assisted selection and introgression of trait of interest
• SSR/SNP marker development for varietal fingerprinting and hybrid purity testing (agarose gel based)
• Validated molecular markers for different disease tolerance, quality and Morphological Traits

• Event identity, trait purity, event zygosity testing
• Transgenic contamination with a Limit of Detection (LOD) of 0.01%

At I &B Seeds Pvt Ltd, we have established seed health testing for vegetable seeds based on the International Seed Health Initiative for Vegetable crops ( ISHI-Veg ) protocols. Currently we are offering services for pepper and tomato seed testing for viral and bacterial pathogens. Tests for other economically important pathogens associated with seeds of other crops can be customised as needed.

Development of commercially popular hybrids depends upon the availability of homozygous parental lines in any crop species. In conventional breeding program homozygous lines are developed by repetitive self-pollination for 6-8 generations which might take 3-4 years depending upon the crop species.

Availability of optimized doubled haploid protocols circumvents this problem and homozygous parental lines could be developed in two generations within a year. We have successfully developed high throughput protocols for doubled haploid production in sweet and hot peppers. With a turnaround time of less than a year we could develop homozygous lines from a hybrid of customer’s choice. We are in the process of developing optimized protocols for haploid production of cucumbers.